Table 2 Binding affinities for MurD ligands.

From: Ligand-driven conformational changes of MurD visualized by paramagnetic NMR

Ligand

Condition

Kd [μM]

NMR

ITC

Mg2+

 

NDa

–

AMP-PNP

 

630 ±40

300 ± 75

AMP-PNP

Mg2+

8.4 ± 1.1

19.8 ±1.7

UMA

 

930 ± 30

–

UMA

Mg2+, AMP-PNP

28.5 ± 5.0

62.9 ± 4.7

d-Glu

 

NDa

–

d-Glu

Mg2+, AMP-PNP, UMA

NDa

–

d-Glu

Mg2+, ADP, UMA

–

–

Compound 1

 

8.9 ± 2.4

–

Compound 2

 

110 ± 17

116 ± 10

  1. aNot detected: Binding was too weak to be detected.
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