Figure 8 | Scientific Reports

Figure 8

From: Upregulation of Ih expressed in IB4-negative Aδ nociceptive DRG neurons contributes to mechanical hypersensitivity associated with cervical radiculopathic pain

Figure 8

Increased expression of HCN1 and HCN3, but not HCN2 in IB4− small diameter DRG neurons in CRP rats compared to controls.

(a) Double immunofluorescence staining of DRG neurons with IB4 (red) and HCN1 (green, upper panels), HCN2 (green, middle panels), HCN3 antibody (green, lower panels) in CRP and control rats. (b,d) Quantitative summary from double immunofluorescence experiments showing that in CRP rats, HCN1 (b) and HCN3 (d) immunoreactivity in IB4− small diameter DRG neurons was increased in comparison with control rats. The percentage of IB4− small diameter DRG neurons expressing HCN1 immunoreactivity in all DRG neurons is shown. (c) HCN2 immunoreactivity in IB4− small diameter DRG neurons did not show obvious alteration in CRP rats compared to controls. All data are represented as mean ± SEM. Scale bars represent 50 μm. *P < 0.05 as compared to control rats.

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