Figure 8

Changes in serum levels of IFN-γ, IL-2 and IL-4, as well as expressions of Foxp-3 and CTLA-4 on splenic CD4⁺CD25⁺ Tregs at 24 hours after treatment with T-peptide in septic mice.

Treatment with T-peptide could markedly elevate serum level of IFN-γ (a), dually regulate IL-2 level (b), while down-regulate the level of IL-4 (c) and modulate the balance of IFN-γ/IL-4 (d) at 24 hours in a dose-dependent manner. The expressions of Foxp-3(e) and CTLA-4(f) on splenic CD4⁺CD25⁺ Tregs were down-regulated at 24 hours after treatment with T-peptide in septic mice. Data were represented as mean ± standard deviation (SD) and analyzed by software of SPSS 17.0 with a one-way ANOVA, n = 4 per group. Statistically significant difference as compared with the control group, (*)P < 0.05, (**)P < 0.01; Statistically significant difference as compared with the CLP + 0.9% NS group, (#)P < 0.05, (##)P < 0.05; Statistically significant difference as compared with the CLP + 0.25 mg/kg T-peptide group, (&)P < 0.05, (&&)P < 0.05; Statistically significant difference as compared with the CLP +1 mg/kg T-peptide group, (@)P < 0.05.