Figure 3

Validation of iTRAQ analysis.

(A) Significant activates pathways obtained by comparing modulated proteins obtained by iTRAQ analysis employing functional annotations according to the String 9.0 software. Small numbers on the right of each bar are the modulated protein involved in each process. (B) Western blot analysis of Lamin A/C, Vimentin and Superoxide dismutase 2 (SOD-2). Tubulin was used as housekeeping. On the left molecular weight of each protein is shown. On the bottom the group’s source of mesenchymal stem cells used. The gels have been run under the same experimental conditions. (C) Vinculin gene expression using real-time reverse transcriptase PCR (qRT-PCR) analysis normalized by expression of HPRT gene used as housekeeping.