Figure 3

Localization of tagged forms of TbEPT and TbCEPT in the same cell.
Panel (A) GFP-tagged TbEPT (top panels) and TbCEPT (bottom panels) were transiently expressed in T. brucei procyclic forms and co-stained with MAb414, a nuclear pore complex marker. Panel (B) GFP-tagged TbEPT was transiently expressed in procyclic forms stably expressing 3xcmyc-TbCEPT and cells were immunostained with anti-cmyc antibody and fluorescence-conjugated goat anti-mouse antibody. DNA in the composite pictures was stained with DAPI. DIC, differential interference contrast.