Figure 5 | Scientific Reports

Figure 5

From: Phosphatidylethanolamine and phosphatidylcholine biosynthesis by the Kennedy pathway occurs at different sites in Trypanosoma brucei

Figure 5

Fractionation of TbCEPT and TbEPT by gradient centrifugation.

Panel (A) The membranes and organelles fraction, obtained after breaking up the cells followed by a centrifugation step, was further separated using a linear Nycodenz gradient. Fractions collected from the top of the gradient were then analysed for the presence of tagged TbCEPT and TbEPT. Panel (B) SDS-PAGE and Western blot analysis of odd-numbered fractions from a mixed culture of trypanosomes expressing 3xcmyc-CEPT or EPT-3xHA. Proteins were detected using antibodies against HA, cmyc, BiP (as ER marker), mtHSP70 (as mitochondrial marker), MAb414 (as nuclear envelope marker) and aldolase (as glycosomal marker).

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