Table 1 Compendium of aptamers generated by selection against PL45 cells.
From: Development of a panel of DNA Aptamers with High Affinity for Pancreatic Ductal Adenocarcinoma
Aptamers | Sequence | Relative abundance | Kd ± s.d. (nM) at 4 °C | Kd ± s.d. (nM) at 37 °C |
|---|---|---|---|---|
PL1 | 5′-CGC TCG GAT GCC ACT ACA GTG CTA ATC TCA AGG GTC GTT CCC GAT CAC CGA GTC TGA GGC TGG-3′ | 8.88% | 28.4 ± 3.6 | 24.9 ± 3.6 |
PL2 | 5′-CGC TCG GAT GCC ACT ACA GGA ACT AAC ACA CTA CTG AAC CGT GCT CAC CGA GTC TGA GGC TGG-3′ | 8.24% | 8.3 ± 2.5 | 20.9 ± 3.5 |
PL3 | 5′-CGC TCG GAT GCC ACT ACA GCA CTC ACC TCA AGG GTT CCG TGT CAC CGA GTC TGA GGC TGG-3′ | 7.55% | 16.2 ± 3.0 | 23.1 ± 5.3 |
PL4 | 5′-CGC TCG GAT GCC ACT ACA GGG ACT AAG CAC ACT ACT GTT CAC GGT CAC CGA GTC TGA GGC TGG-3′ | 5.51% | 16.6 ± 6.5 | 11.9 ± 1.4 |
PL5 | 5′-CGC TCG GAT GCC ACT ACA GCC AGC GTG GAT ATG GGT TCC ACT GGT CAC CGA GTC TGA GGC TGG-3′ | 2.75% | 55.3 ± 4.4 | 65.5 ± 18.1 |
PL6 | 5′-CGC TCG GAT GCC ACT ACA GTA CAC ACT GGT CTC AAG GGT GTG AGT CAC CGA GTC TGA GGC TGG-3′ | 2.49% | 47.3 ± 16.0 | 178.9 ± 30.1 |
PL7 | 5′-CGC TCG GAT GCC ACT GTT GAG GTG TAT TGT ACA CGT GGG GTT ACA CAC CGA GTC TGA GGC TGG-3′ | 3.00% | 43.3 ± 13.8 | 30.0 ± 11.2 |
PL8 | 5′-CGC TCG GAT GCC ACT ACA GCA TAT ATC CTC CCC CCA TGC GTG GTC ACC GAG TCT GAG GCT GG-3′ | 2.09% | 60.9 ± 15.5 | 26.1 ± 6.5 |
