Figure 5 | Scientific Reports

Figure 5

From: Intravenous multipotent adult progenitor cell treatment decreases inflammation leading to functional recovery following spinal cord injury

Figure 5

Microarray analysis reveals that MAPC treatment 1 dpi modulates immune related genes and pathways at the injury site four days post SCI.

(a) Volcano plot showing differentially expressed genes at the injury site following MAPC treatment compared to saline treatment. Black circles p < 0.05, grey circles p > 0.05, t-statistic with Bayesian adjusted denominator (b) Principal component analysis of each individual animal’s expression of genes significantly modulated by MAPC treatment compared to saline treatment. (c) Dendrogram of unsupervised agglomerative hierarchical cluster analysis and gene expression heat map of genes significantly modulated by MAPC treatment compared to saline treatment. (d) Upstream regulators significantly modulated by MAPC treatment using Ingenuity pathway analysis. * denotes a bias corrected z-score. (e) Real-time qPCR verification of NOS2, Ly6C and MMP7 gene expression changes. n = 6 per group, 3 biological replicates per animal. ****p < 0.0001 when compared to Saline treatment. Two-way ANOVA, Fisher’s Least Significant Difference post-hoc test.

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