Figure 2
From: Mutant cohesin affects RNA polymerase II regulation in Cornelia de Lange syndrome
Analysis of Pol II recruitment at the promoter regions by ChIP-qPCR using anti-Pol II antibody (CTD domain).
(A) Pol II recruitment decreases in CdLS cell lines compared to control cell lines at the promoter regions of downregulated genes in SMC1A-mutated CdLS cell lines (B) Pol II enrichment does not significantly change in CdLS cell lines at the promoter regions of upregulated genes. I: genes virtually active in all cell types, II: genes preferentially expressed in lymphoblastoid cells and III: our best targets based on ChIP-Seq enrichment. Since no difference was found, control cell line data were pooled. IgG was used as negative control. Results represent three independent ChIP assays normalized to genomic regions without Pol II enrichment. The average values of the experiments and the relative standard errors are shown. *p < 0.05.
