Figure 5
(a–d): Effect of Fra-2 silencing on cell invasion and migration of HPV+ve and HPV−ve TSCC cells. Fra-2 knockdown by specific siRNA leads to alterations in invasion in both HPV−ve (a) and HPV+ve (b) TSCC cells. Matrigel invasion assay of untreated, scrambled siRNA and Fra-2 siRNA treated in TSCC cells. Equal number of cells (1 × 105) were seeded in the Matrigel coated upper chamber. After 24 hours of incubation at 37 °C and 5% CO2, the cells that invaded through the membrane were fixed and stained with Giemsa and images were captured using inverted microscope. Representative fields of view for each well are shown. Cell invasion was quantified by counting cells in six random fields. (c,d) Scratch assay of Fra-2 silencing demonstrated that cell migration was dramatically decreased in experimental groups after Fra-2 transfection compared with untreated and scrambled treated control groups. Inverted microscope images of wound closure at 0 hours, 12 hours and 18 hours in AW13516 (c) and UPCI:SCC90 (d) cells as indicated. Migration rate was calculated by image J software. The data in bar diagram are expressed as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 & ****p < 0.0001.
