Figure 1
From: Crosstalk between SDF-1/CXCR4 and SDF-1/CXCR7 in cardiac stem cell migration
Identification of CSCs and detection of CXCR4 and CXCR7 in CSCs.
(A) Identification of c-kit+ CSCs with a purity of 93.6% by flow cytometry. (B) RT-PCR analysis of CXCR4 and CXCR7 mRNA in CSCs. 4T1 cells were used as a positive control. (C) Western blot analysis of CXCR4 and CXCR7 protein in CSCs. 4T1 cells were used as a positive control. (D) RT-PCR analysis of CXCR4 mRNA in CSCs with CXCR4 siRNA. (E) RT-PCR analysis of CXCR7 mRNA in CSCs with CXCR7 siRNA. (F) Western blot analysis of CXCR4 and CXCR7 protein in CSCs with CXCR4 siRNA and CXCR7 siRNA. *P < 0.05 versus control.
