Figure 7
From: Formononetin promotes angiogenesis through the estrogen receptor alpha-enhanced ROCK pathway
Inhibition of ROCK-II expression by ROCK-II siRNA abolished the activation of ROCK and MMP2/9 signaling pathways in HUVECs.
(A) HUVECs were transfected with either non-specific siRNA as control or specific ROCK-II siRNA for 2 days followed by 50 μM formononetin treatment for 30 or 60 min. The protein levels of ROCK-II, MMP2, MMP9, P-MYPT1, P-LIMK1, P-cofilin and P-MLC2 were analyzed by using Western blotting. (B) Statistical analysis of the protein levels of MMP2 and MMP9 in HUVECs transfected with ROCK-II siRNA in the presence of 50 μM formononetin. (C) Statistical analysis of the protein levels of P-MYPT1, P-LIMK1, P-cofilin and P-MLC2 in HUVECs transfected with ROCK-II siRNA in the presence of 50 μM formononetin. Results are expressed as a percentage of control (means ± SD, n = 3), *p < 0.05, **p < 0.01 vs. control.
