Figure 8
From: Formononetin promotes angiogenesis through the estrogen receptor alpha-enhanced ROCK pathway
Formononetin induced the activations of ROCK and MMP2/9 pathways via ERα.
(A) HUVECs were transfected with either non-specific siRNA as control of specific ERα siRNA for 2 days followed by 50 μM formononetin treatment for 30 or 60 min. The protein levels of ERα, MMP2, MMP9, P-MYPT1, P-LIMK1, P-cofilin and P-MLC2 were analyzed by using Western blotting. (B) Statistical analysis of the protein levels of MMP2 and MMP9 in HUVECs transfected with ERα siRNA in the presence of 50 μM formononetin. (C) Statistical analysis of the protein levels of P-MYPT1, P-LIMK1, P-cofilin and P-MLC2 in HUVECs transfected with ERα siRNA in the presence of 50 μM formononetin. Results are expressed as percentages of controls (mean ± SD; n = 3), *p < 0.05, **p < 0.01 vs. control.
