Figure 9
From: Formononetin promotes angiogenesis through the estrogen receptor alpha-enhanced ROCK pathway
Inhibition of ER activation by ICI 182,780 abolished the interaction between ERα and ROCK-II induced by formononetin.
(A–D) HUVECs were treated with 0.1% DMSO or 50 μM formononetin for 15, 30, 45 and 60 min. (E–H) HUVECs were pretreated with 10 μM ICI 182,780 for 2 h, followed by washout and treatment with 0.1% DMSO or 50 μM formononetin for 30 and 60 min. HUVECs protein extracts were immunoprecipitated with anti-ERα antibody and the immunoprecipitations (IPs) were assayed for co-immunoprecipitation of ROCK-II with Western blotting. HUVECs total protein was directly assayed with western blotting analysis as controls. Results are expressed as percentage of control in mean ± (n = 3), *p < 0.05, **p < 0.01 vs. control.
