Table 1 Primers used for quantitative real time PCR.

From: Fibulin-4 deficiency increases TGF-β signalling in aortic smooth muscle cells due to elevated TGF-β2 levels

Genes

Forward primers

Reverse primers

Fibulin-4

5′-GGGTTATTTGTGTCTGCCTCG-3′

5′-TGGTAGGAGCCAGGAAGGTT-3′

SMA

5′-GTCCCAGACATCAGGGAGTAA-3′

5′-TCGGATACTTCAGCGTCAGGA-3

TGF-β1

5′-CAACAATTCCTGGCGTTACC-3′

5′-TGCTGTCACAAGAGCAGTGA-3′

TGF-β2

5′-CCGCCCACTTTCTACAGACCC-3′

5′-GCGCTGGGTGGGAGATGTTAA-3′

  1. Forward and reverse primers are displayed for each gene from 5′ to 3′.
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