Figure 3 | Scientific Reports

Figure 3

From: Osteoarthritis-like pathologic changes in the knee joint induced by environmental disruption of circadian rhythms is potentiated by a high-fat diet

Figure 3

Chronic circadian disruption and HFD increase activation of catabolic signaling pathways in the mouse knee joint.

(a,c) Representative immunohistochemical staining images in knee joint sections. Pictures of immunostaining were obtained under 200X magnification (n = 4 per group), representing medial femoral condyle and tibial plateau. For each immuostain, a negative control without primary antibody was included. Scale bars, 50 μm. (c,d) Quantitative histomorphometric analyses. Percent of positively stained cells in articular cartilage and calcified cartilage were counted using ImageJ (NIH, Bethesda, MD). For each group n = 4. Values are presented as mean ± SD (Comparing non-shifted ND mice to shifted ND mice: *P < 0.05; **P < 0.01; comparing non-shifted ND mice to shifted HFD mice: §§P < 0.01; §§§P < 0.001; comparing shifted ND mice to shifted HFD mice: #P < 0.05 ##P < 0.01).

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