Figure 1
p53 expression increased in HPF-a either cultured in conditioned medium of AMOs derived from silicosis patients or directly exposed to SiO2.
(A) Axial high-resolution CT sections of patients at different stages of silicosis. (B) ELISAs showing that the MCP-1 level in both the BAL fluid and the AMO culture medium from silicosis patients was significantly higher than that in samples from healthy donors. p < 0.05, n = 5. (C) Representative Western blot showing the effect of AMO-conditioned medium derived from patients or healthy donors on p53 expression in HPF-a. (D) Densitometric analyses of p53 expression from five healthy donors and five silicosis patients suggested that the p53 protein level in HPF-a cultured in medium from the patient group was significantly higher than the level in HPF-a cultured in medium from the healthy group. *p < 0.05. (E) ELISAs showing that SiO2 induced a rapid and sustained increase in MCP-1 expression in the supernatant of cultured AMOs from healthy donors. *p < 0.05 vs the 0 h group, n = 5. (F) Representative Western blot showing that SiO2 induced p53 expression in HPF-a in a dose-dependent manner. (G) Densitometric analyses of p53 (n = 5). *p < 0.05 vs the control group.
