Figure 1
From: Revisiting the role of phospholipases C in virulence and the lifecycle of Mycobacterium tuberculosis
Construction of M. tuberculosis H37RvplcABC KO (H37RvΔPLC).
(A) Schematic representation of genomic organization of plc genes in M. tuberculosis H37Rv wild type and H37RvΔPLC strains ; (B) AvrII restriction fragment profiles of M. tuberculosis WT and KO strains separated by agarose gel electrophoresis; (C) Pattern obtained from genomic DNAs digested with AvrII and hybridized with a probe specific for the plcC downstream region; Lanes: 1 (second lane from left), negative control (pYUB412 vector); 2, positive control pYUB412::plcABC; 3 and 4, M. tuberculosis H37Rv WT, 5 and 6, M. tuberculosis H37RvΔplcABC; 7, M, Smart Ladder (Eurobio).
