Figure 3
From: Revisiting the role of phospholipases C in virulence and the lifecycle of Mycobacterium tuberculosis
Phagosomal rupture by M. tuberculosis mutants.
Capacity of different M. tuberculosis strains and mutants to induce phagosomal rupture inTHP-1 cells, monitored by CCF-4 staining and flow cytometric analysis. Non infected (gray), infected (MOI = 1:10) with different strains of M. tuberculosis (purple) using a recently developed approach23. Results shown are representative of 2 independent experiments. The shift towards blue emission (447 nm) of CCF-4 is due to the inhibition of FRET and is proportional to the mycobacteria-induced phagosomal rupture/cytosolic access.
