Figure 3 | Scientific Reports

Figure 3

From: Position-specific automated processing of V3 env ultra-deep pyrosequencing data for predicting HIV-1 tropism

Figure 3

Example of PyroVir analysis for predicting HIV-1 quasispecies coreceptor usage.

RNA was extracted from a plasma sample from an HIV-infected subject and submitted to nested RT-PCR amplification of V3 env, ultra-deep pyrosequencing and PyroVir analysis. Ultra-deep pyrosequencing provided 2,549 analyzable V3 reads, of which 107 were discarded by the biological filter and and 74 by the statistical filter. 2 variants, one accounting for 2.9% and the other for 1% of the quasispecies, were predicted to use CXCR4.

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