Table 2 Characteristics of the six species analyzed for population genetics by MIG-seq.

From: MIG-seq: an effective PCR-based method for genome-wide single-nucleotide polymorphism genotyping using the next-generation sequencing platform

Species

Collection sites

Total no. of input reads for Stacks (min.–max.)

No. of putative loci (Stacks)

No. of SNPs

A

B

Nameko mushroom (Pholiota microspora)

Cultivated varieties from Miyagi Prefecture

Wild strains from Fukushima Prefecture

1,814,441 (138,636–301,781)

505

31

Calanoida copepod (Eodiaptomus japonicus)

Takashima, Shiga N35°15′, E136°4′

Ono, Hyogo N34°50′, E134°53′

3,195,755 (273,699–494,513)

3,212

115

Japanese common sea cucumber (Apostichopus japonicus)

Red types from Onagawa, Miyagi N38°25′, E141°29′

Green types from Onagawa, Miyagi N38°25′, E141°29′

9,892,527 (998,736–1,497,056)

3,409

125

Predatory sea snail (Laguncula pulchella)

Matsukawa-ura, Fukushima N37°46′, E140°58′

Matsushima, Miyagi N38°19′, E141°8′

7,887,899 (560,753–1,655,007)

4,843

324

Carolina anole (Anolis carolinensis)

Naha, Okinawa N26°13′, E127°41′

Ogasawara Islands N27°5′, E142°11′

6,773,549 (389,990–1,230,227)

2,354

83

Lady’s-slipper orchid (Cypripedium macranthos var. rebunense)

Northern Rebun Island N45°24′, E141°0′

Southern Rebun Island N45°19′, E141°0′

3,869,894 (349,500–650,200)

3,852

209