Figure 4
From: EZH2 is highly expressed in pituitary adenomas and associated with proliferation
Treatment with GSK126 leads to reduced trimethylation of H3K27 in AtT-20 cells.
This was shown to occur in a dose dependent manner (A) within 72 h. Densitometric evaluation of H3K27me3 blot is depicted below. H3 was used as loading control and served for normalization of H3K27me3 data that were compared with solvent treated AtT-20 cells set to 100%. H3K27 trimethylation decreases after 10 μM GSK126 treatment over time (B). Treatment with 10 μM GSK126 for 72 h was repeated in three independent experiments and densitometric evaluation showed a significant (p ≤ 0.05) decrease (C).
