Figure 5
From: EZH2 is highly expressed in pituitary adenomas and associated with proliferation
To prove a possible impact of EZH2 on cell viability and proliferation, AtT-20 cells were treated with GSK126 in a concentration ranging from 10 nM to 100 μM for 72 h.
Values were compared to DMSO treated controls that were set to 100%. MTT assay revealed that cell viability decreased significantly when cells were exposed to a concentration of GSK126 >10 μM (A). However, BrdU assay showed that concentrations of ≥10 μM GSK126 reduced cell proliferation significantly (B). Two tailed paired students t-test was assessed as significant with p < 0.05. The combination of three independent BrdU examinations (C) confirmed a significant decrease of proliferation in AtT-20 cells when treated with 10 μM GSK126 (p < 0.05; Friedmann test with matched values and Dunn´s post test).
