Figure 5
Effect of DMCs on the secretion of Raw264.7 cell supernatant cytokines.
(A) The co-culture of DMCs with Raw264.7 decreased IL-1β secretion at both 24 and 48 h after LPS stimulation, whereas there was no significant difference in IL-1β secretion between contact or non-contact co-culture manner when compared to Raw264.7 alone. (B) The co-culture of DMCs with Raw264.7 decreased IL-6 secretion at both 24 and 48 h after LPS stimulation and there were significant differences in IL-6 secretion between contact and non-contact co-culture manners when compared to Raw264.7 alone. (C) The co-culture of DMCs with Raw264.7 increased IL-10 secretion at both 24 and 48 h after LPS stimulation and there were significant differences in IL-10 secretion between contact and non-contact co-culture manners when compared to Raw264.7 alone at 48 h, whereas no significant difference was observed at 24 h. RAW: Raw264.7 alone without LPS stimulation; RAW + LPS: Raw264.7 alone with LPS stimulation; RAW-DMCs + LPS: mixture contact co-culture of DMCs with Raw264.7 with LPS stimulation; RAW/DMCs + LPS: non-contact co-culture of DMCs with Raw264.7 with LPS stimulation. n = 5 at each group. * indicates Independent-Samples t-test p-values < 0.05.
