Figure 6 | Scientific Reports

Figure 6

From: Non-cultured dermal-derived mesenchymal cells attenuate sepsis induced by cecal ligation and puncture in mice

Figure 6

Co-culture with DMCs inhibited apoptosis in macrophages.

(A) Raw264.7 were stained with Annexin-V-/Propidiumiodid and analyzed for apoptosis in the presence or absence of LPS stimulation. Significantly less apoptosis was observed in co-cultured Raw264.7 cells (Annexin-V-positive, PI-negative cells). RAW: Raw264.7 alone without LPS stimulation; RAW + LPS: Raw264.7 alone with LPS stimulation; RAW/DMCs + LPS: non-contact co-culture of DMCs with Raw264.7 with LPS stimulation. (B) Peritoneal macrophages apoptosis. PM: peritoneal macrophages alone without LPS stimulation; PM + LPS: peritoneal macrophages alone with LPS stimulation; PM/DMCs + LPS: non-contact co-culture of DMCs with peritoneal macrophages with LPS stimulation. Results represent 3 independent experiments.

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