Figure 7
A co-culture experiment showed that DMCs can increase the migration activity of peritoneal macrophages and Raw264.7 macrophages in the presence or absence of LPS.
DMCs were co-cultured with macrophages in transwell (8 μm) in non-contact condition. The migration activity of macrophages was measured at 6 h after LPS (1 μg/ml) stimulation. Cells migrating through the membrane were stained by hematoxylin after fixed with 4% paraformaldehyde. (A) Raw264.7 macrophages migration. RAW: Raw264.7 alone without LPS stimulation; RAW + LPS: Raw264.7 alone with LPS stimulation; RAW/DMCs: non-contact co-culture of DMCs with Raw264.7 without LPS stimulation; RAW/DMCs + LPS: non-contact co-culture of DMCs with Raw264.7 with LPS stimulation. (B) peritoneal macrophages migration. PM: peritoneal macrophages alone without LPS stimulation; PM + LPS: peritoneal macrophages alone with LPS stimulation; PM/DMCs: non-contact co-culture of DMCs with peritoneal macrophages without LPS stimulation; PM/DMCs + LPS: non-contact co-culture of DMCs with peritoneal macrophages with LPS stimulation. Results represent 3 independent experiments. Bar represent 200 μm.
