Figure 8
DMCs enhanced phagocytosis of macrophages.
(A) macrophages Raw264.7 were co-cultured with DMCs for 4 h, then stimulated by LPS (1 μg/ml). And then Raw264.7 cells were incubated with fluorescent latex beads for 12 h. Phagocytosis ability were analyzed by fluorescence microscope and FACS. RAW + LPS: Raw264.7 alone with LPS stimulation; RAW/DMCs + LPS: non-contact co-culture of DMCs with Raw264.7 with LPS stimulation. (B) Peritoneal macrophages were treated as Raw264.7, then incubated with fluorescent latex beads for 4 h. Phagocytosis ability were analyzed by fluorescence microscope. Peritoneal macrophages were identified using a PE anti-mouse F4/80 antibody and were measured by FACS. PM + LPS: peritoneal macrophages alone with LPS stimulation; PM/DMCs + LPS: non-contact co-culture of DMCs with peritoneal macrophages with LPS stimulation. (C) Phagocytosis analysis in vivo: The CLP mice were made and treated with saline or DMCs as described above. One day after CLP, fluorescent latex beads were injected into the peritoneal cavity of CLP mice. Mice were sacrificed 2 hours after injection. Peritoneal cells were stained by PE anti-mouse F4/80 and analyzed by FACS. Results represent 3 independent experiments. Bar represent 100 μm.
