Figure 5
Effects of RA-XII on the expressions of molecules in various signaling pathways in breast cancer cells.
Representative blots revealed the expressions of the signaling molecules involved in (A) FAK/pSRC, (B) NF-κB/IκB and (C) PI3K/AKT in 4T1 cells treated with RA-XII at 100 nM at various time-points. Representative blots also showed the expressions of the signaling molecules involved in (D) FAK/pSRC, (E) NF-κB/IκB, (F) PI3K/AKT and (G) MAPK signaling pathways in 4T1 cells treated with RA-XII at 50 and 100 nM for 24 hours. Whole cell lysate was subjected to Western blotting. (H) Representative blots showed the expressions of the signaling molecules involved in EGFR signaling in 4T1 cells treated with RA-XII at 50 and 100 nM for 6 hours and EGF (10 ng/mL) for 2 hours, prior to cytoplasmic protein extraction. The cropped blots have been obtained under the same experimental conditions and the full-length blots were shown in Supplementary information. The histograms showed the quantified results of the expressions of the target proteins involved in various pathways which were normalized with corresponding β-actin protein expression and expressed as folds of control (mean + SD of 3–4 independent experiments). Statistical differences were determined by One-way ANOVA, followed by Dunnett Test, with *p < 0.05, **p < 0.01, ***p < 0.001 against untreated controls.
