Table 1 Select Fa and CI values of top six combinations with order-of-addition.
From: Identification of Synergistic, Clinically Achievable, Combination Therapies for Osteosarcoma
Select Fa and CI values of top six combinations with order-of-addition. | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Tx1 | Tx2 | Conc (ng/ml) | Order of Addition | MNNG/HOS | 143B | MG63 | U-2 OS | Saos2 | FA | CI | P-value, N = 10 (FA; CI) | ||||||||
Tx1 | Tx2 | FA | CI | FA | CI | FA | CI | FA | CI | FA | CI | (mean ± SEM) | (mean ± SEM) | Con vs. 1→2 | Con vs. 2→1 | 1→2 vs. 2→1 | |||
Bortezomib | Panobinostat | 12 | 14 | Concurrent | 1.00 | 0.43 | 1.00 | 0.38 | 1.00 | 0.60 | 1.00 | 0.12 | 0.95 | 0.60 | 0.99 ± 0.01 | 0.44 ± 0.08 | 0.016; <0.001 | 0.141; 0.111 | 0.228; <0.001 |
Tx1 → Tx2 | 0.95 | 0.85 | 1.00 | 0.94 | 0.99 | 0.88 | 0.95 | 0.42 | 0.78 | 0.64 | 0.94 ± 0.02 | 0.76 ± 0.06 | |||||||
Tx2 → Tx1 | 0.94 | 0.54 | 1.00 | 0.36 | 0.96 | 0.44 | 1.00 | 0.07 | 0.99 | 0.25 | 0.98 ± 0.01 | 0.34 ± 0.06 | |||||||
Bortezomib | Romidepsin | 12 | 200 | Concurrent | 1.00 | 0.35 | 1.00 | 0.91 | 1.00 | 0.59 | 1.00 | 0.12 | 1.00 | 0.48 | 1.00 ± 0.00 | 0.50 ± 0.09 | 0.010; 0.001 | 0.040; 0.010 | 0.026; <0.001 |
Tx1 → Tx2 | 0.97 | 0.77 | 1.00 | 0.82 | 0.99 | 0.83 | 0.98 | 0.41 | 0.93 | 0.99 | 0.98 ± 0.01 | 0.77 ± 0.07 | |||||||
Tx2 → Tx1 | 0.99 | 0.36 | 1.00 | 0.39 | 1.00 | 0.11 | 1.00 | 0.07 | 1.00 | 0.51 | 1.00 ± 0.00 | 0.27 ± 0.06 | |||||||
Carfilzomib | Panobinostat | 30 | 14 | Concurrent | 0.98 | 0.33 | 1.00 | 0.36 | 1.00 | 0.39 | 1.00 | 0.05 | 1.00 | 0.23 | 1.00 ± 0.00 | 0.28 ± 0.07 | 0.051; <0.001 | 0.002; 0.004 | 0.002; 0.090 |
Tx1 → Tx2 | 0.91 | 0.61 | 1.00 | 0.58 | 1.00 | 0.62 | 0.99 | 0.21 | 0.98 | 0.54 | 0.98 ± 0.01 | 0.52 ± 0.08 | |||||||
Tx2 → Tx1 | 0.60 | 0.78 | 0.90 | 0.53 | 0.81 | 1.18 | 0.85 | 0.75 | 1.00 | 0.20 | 0.83 ± 0.04 | 0.73 ± 0.13 | |||||||
Carfilzomib | Romidepsin | 30 | 200 | Concurrent | 0.99 | 0.21 | 1.00 | 0.49 | 1.00 | 0.37 | 1.00 | 0.08 | 1.00 | 0.60 | 1.00 ± 0.00 | 0.35 ± 0.07 | 0.010; 0.003 | 0.039; 0.459 | 0.659; 0.022 |
Tx1 → Tx2 | 0.93 | 0.61 | 1.00 | 0.65 | 1.00 | 0.66 | 0.96 | 0.53 | 0.97 | 0.65 | 0.97 ± 0.01 | 0.62 ± 0.08 | |||||||
Tx2 → Tx1 | 0.91 | 0.55 | 1.00 | 0.50 | 1.00 | 0.12 | 0.98 | 0.34 | 1.00 | 0.31 | 0.98 ± 0.01 | 0.34 ± 0.08 | |||||||
MK1775 | Ixabepilone | 980 | 80 | Concurrent | 0.96 | 0.85 | 0.98 | 0.49 | 0.87 | 1.67 | 0.93 | 0.50 | 0.97 | 0.96 | 0.94 ± 0.01 | 0.89 ± 0.17 | 0.003; 0.446 | 0.070; 0.113 | 0.014; 0.054 |
Tx1 → Tx2 | 0.89 | 1.29 | 0.96 | 0.43 | 0.82 | 1.42 | 0.82 | 0.99 | 0.94 | 0.79 | 0.88 ± 0.02 | 1.02 ± 0.16 | |||||||
Tx2 → Tx1 | 0.93 | 0.77 | 0.97 | 0.36 | 0.88 | 0.75 | 0.86 | 0.58 | 0.96 | 0.98 | 0.92 ± 0.01 | 0.69 ± 0.12 | |||||||
MK1775 | Romidepsin | 980 | 200 | Concurrent | 0.95 | 0.80 | 0.97 | 1.42 | 0.94 | 0.64 | 0.99 | 0.16 | 0.99 | 1.28 | 0.97 ± 0.01 | 0.86 ± 0.18 | 0.144; 0.277 | 0.007; 0.024 | 0.303; 0.066 |
Tx1 → Tx2 | 0.87 | 1.46 | 0.99 | 0.23 | 0.92 | 0.80 | 0.94 | 0.65 | 0.99 | 0.46 | 0.94 ± 0.02 | 0.73 ± 0.22 | |||||||
Tx2 → Tx1 | 0.85 | 1.17 | 0.79 | 3.36 | 0.77 | 1.53 | 0.93 | 0.49 | 0.99 | 1.58 | 0.86 ± 0.03 | 1.62 ± 0.41 | |||||||
