Figure 5 | Scientific Reports

Figure 5

From: The role of mesenchymal stem cells in promoting the transformation of androgen-dependent human prostate cancer cells into androgen-independent manner

Figure 5

Androgen could effectively inhibited the expression of TGF-β in MSCs.

(A) ELISA assay was employed to detect the expression of TGF-β in MSCs DMEM culture medium with or without fetal bovine serum (FBS). (B,C) The MSCs culture medium (DMEM with 10% FBS) treated with 10% charcoal (CM1) was used to observe their function to induce androgen-independent manner in LNCaP cells compared with the culture medium untreated with 10% charcoal (CM2). CCK8 and PI/Annexin V-FITC assay was used to examined the cell viability and apoptosis of LNCaP cells in an androgen-deprivation (AD) condition (*P < 0.05).

Back to article page