Figure 4
From: Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES
Kinetics of CCR5 proteolysis by subtilisin.
Representative time courses for the proteolysis of CCR5 produced with and without GroEL-GroES added to the cell-free reaction were compared. Proteolytic reactions supplemented with bovine albumin were also performed as controls. The molar concentration of albumin was the same as the concentration of the added GroEL-GroES. The fit of the kinetic data to a 2-phase exponential equation (
) is shown to describe the different CCR5 proteolytic processes.
