Table 1 Kinetic parameters describing the cell-free translation and folding of CCR5, its proteolysis by subtilisin and its ligand-binding capacity with or without the addition of chaperonins and/or their inhibitor DMPHBA.
From: Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES
Events | Kinetic parameters | No addition | DMPHBA | GroEL-GroES | GroEL-GroES+DMPHBA | GroEL |
|---|---|---|---|---|---|---|
Translation | ktrans/min−1 | (3.0 ± 0.8) × 10−2 | (4.6 ± 0.9) × 10−2 | (5.2 ± 1.0) × 10−2 | (3.6 ± 0.7) × 10−2 | (4.1 ± 0.8) × 10−2 |
Folding | kfold/min−1 | (8.2 ± 1.2) × 10−3 | (9.6 ± 1.6) × 10−3 | (3.0 ± 0.5) × 10−1 | (9.1 ± 1.5) × 10−3 | (7.0 ± 1.2) × 10−2 |
Proteolysis | kpro1/min−1 | 2.8 ± 1.2 | N/D | 3.9 ± 2.0 | N/D | 2.3 ± 1.0 |
|  | kpro2/min−1 | (4.3 ± 0.8) × 10−2 | N/D | (1.2 ± 0.2) × 10−2 | N/D | (2.3 ± 0.5) × 10−2 |
Ligand binding | ka/M−1s−1 | (4.7 ± 1.0) × 103 | N/D | (4.5 ± 0.8) × 103 | N/D | (4.6 ± 0.9) × 103 |
|  | kd/s−1 | (4.6 ± 1.0) × 10−4 | N/D | (2.0 ± 0.4) × 10−4 | N/D | (2.5 ± 0.5) × 10−4 |
|  | KD/M | (9.7 ± 0.4) × 10−8 | N/D | (4.4 ± 0.2) × 10−8 | N/D | (5.5 ± 0.2) × 10−8 |
