Figure 4
Increased plasticity of the CENP-A dimerization interface is a dominant mode of motion.
(A) 2D projection of the top principal component of the H3 nucleosome onto the representative structure, viewed from the side of the DNA pseudo-dyad, highlights mobility in the H2A’ acidic patch restrained by H3′ and a lack of movement in H3′ loop 1. The inset displays the single-unit motion of the H3 dimerization interface. (B) 2D projection of the top principal component onto the representative structure for the CENP-A nucleosome highlights mobility in the H2A’ acidic patch unrestrained by CENP-A’ and enhanced fluctuations in CENP-A’ loop 1. The inset displays the shearing and pinching motion of the CENP-A dimerization interface. Structure figures rendered in VMD.
