Figure 1 | Scientific Reports

Figure 1

From: Physiologically generated presenilin 1 lacking exon 8 fails to rescue brain PS1−/− phenotype and forms complexes with wildtype PS1 and nicastrin

Figure 1

PS1∆exon8 is expressed as early as embryonic day 10.

(A) Genotyping analysis of embryos by PCR for the human PS1∆exon8 transgene. (B) Genotyping results for murine PS1. The upper band corresponds to the PS1(+) allele while the bottom band corresponds to the mutant, KO allele. (C) PS1∆exon8 mRNA expression from embryonic mouse brain extracts by RT-PCR at E10 and E16. Expression is seen as early as E10. (D) Immunoblot of embryonic mouse brain extracts at E10 and E16 and adult mouse brain hippocampal extracts at 18 months of age using monoclonal human specific anti-N-terminus PS1, NT.1 antibody to confirm transgene protein expression. The bottom immunoblot is an actin loading control.

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