Figure 2 | Scientific Reports

Figure 2

From: Physiologically generated presenilin 1 lacking exon 8 fails to rescue brain PS1−/− phenotype and forms complexes with wildtype PS1 and nicastrin

Figure 2

PS1∆exon8 does not rescue embryonic PS1 KO lethality.

(A) E16 hPS1∆exon8(−)/mPS1 KO embryo exhibits a shortened rostro-caudal body axis and tail and intracranial hemorrhaging. (B) hPS1∆exon8(+)/mPS1 KO embryo resembles the hPS1∆exon8(−)/mPS1 KO embryo exhibiting a shortened rostro-caudal body axis and tail and intracranial hemorrhage. (C) One copy of murine PS1, hPS1∆exon8(+)/mPS1(+/−), results in normal development. (D) Ntg embryo shows normal development as well. Sections stained with hematoxylin-eosin from the embryos above show intracranial hemorrhaging in mPS1 KO ((E,I), higher magnification) and hPS1∆exon8(+)/mPS1 KO ((F,J), higher magnification), in the intermediate zone (IZ) next to the left lateral ventricle (arrow), while there is no detectable hemorrhage in hPS1∆8(+)/mPS1(+/−) ((G,K), higher magnification) or Ntg ((H,L), higher magnification). Scale bar in (E–H), 500  μm and (I–L), 200  μm. CP = cortical plate, IZ = intermediate zone, VZ = ventricular zone.

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