Figure 4 | Scientific Reports

Figure 4

From: Physiologically generated presenilin 1 lacking exon 8 fails to rescue brain PS1−/− phenotype and forms complexes with wildtype PS1 and nicastrin

Figure 4

FAD Dutch APP/PS1∆exon8 bigenic mice do not display extracellular amyloid plaques.

APP species were visualized using the monoclonal 6E10 antibody. (A) PS1∆exon8 expression on its own does not exhibit human APP immunoreactivity. (B) FAD Dutch APP mice (18 months-old) exhibit intracellular APP/Aβ-like immunoreactivity. (C) FAD Dutch APP/PS1∆exon8 mice (18 months-old) also exhibit intracellular APP/Aβ-like immunoreactivity. (D) In contrast, another early-onset AD PS1 mutation, PS1∆exon9, when crossed with FAD Dutch APP, does lead to extracellular plaques. (E) Higher magnification of PS1∆exon8 in (A), showing no APP immunoreactivity. (F) Higher magnification of the intracellular APP/Aβ-like immunoreactivity of FAD Dutch APP (APP-Aβ-LIR; arrow) in (B). (G) Higher magnification of FAD Dutch APP/PS1∆exon8 in (C), showing the intracellular APP/Aβ-like immunoreactivity (APP-Aβ-LIR; arrow). (H) Higher magnification of the FAD Dutch APP/PS1∆exon9 plaque seen in (asterisk) (D) All images are from the hippocampus. Scale, (A–D), 2 mm and (E–H), 200 μm.

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