Figure 5 | Scientific Reports

Figure 5

From: Physiologically generated presenilin 1 lacking exon 8 fails to rescue brain PS1−/− phenotype and forms complexes with wildtype PS1 and nicastrin

Figure 5

HA- and FLAG-tagged PS1 and PS1∆exon8 are localized to the endoplasmic reticulum (ER).

HEK 293T cells were transfected with the same construct combinations described in the Results section. After 24 hrs, cells were fixed in 4% paraformaldehyde and immunostained for calnexin (green), a marker for the ER (left panel) and for human PS1 (red, middle panel) showing that the HA- and FLAG-tags do not disrupt normal PS1 colocalization indicated by white arrows (right panel) compared to the Empty Vector and No Transfection conditions which contain endogenous levels of human PS1. (A–C) Empty Vectors. (D–F) HA-PS1 and FLAG-PS1. (G–I) HA-PS1∆exon8 and FLAG-PS1∆exon8. (J–L) No Transfection (M–O). HA-PS1 and FLAG-PS1∆exon8. (P–R) FLAG-PS1 and HA-PS1∆exon8. Scale bar, 200 μm. See supporting material in Supplemental Fig. 1.

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