Figure 8 | Scientific Reports

Figure 8

From: Physiologically generated presenilin 1 lacking exon 8 fails to rescue brain PS1−/− phenotype and forms complexes with wildtype PS1 and nicastrin

Figure 8

Nicastrin and PS1∆exon8 co-immunoprecipitate in vivo.

Co-IP immunoblots of forebrain lysates of PS1∆exon8-positive mice and controls. Top panel contains blots for nicastrin (NCT) made with an antibody targeting the C-terminus of human nicastrin (amino acids 693–709). Bottom panels are blotted with a monoclonal anti-human PS1 specific antibody that detects full length PS1. Columns from left-to-right include immunoprecipitated lysates using a NCT antibody (N1660; Sigma), IgG negative control (sheep anti-rabbit M-280 Dynabeads), PS1 antibody for positive control (D3931; Cell Signaling) (See also Supplemental Fig. 2) and diluted input 1:10. Note that full–length PS1 bands were only detected in PS1∆exon8 lysates (~45 kDa), except in the IgG negative control. NCT immunoprecipitation detected mostly immature forms of NCT (~115 kDa), as compared to PS1 positive controls and inputs. NCT immunoprecipitation picked up immunoblots in PS1∆exon8 lanes, corresponding to full-length PS1 indicating that these two proteins interact.

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