Figure 5

The yeast mutant K667 complemented by OsCCX2 provide tolerance against excess Fe²⁺, Zn²⁺ and Co²⁺.

Yeast strains transformed with vectors and OsCCX2 were grown in SC-Ura medium for overnight at 30 °C and diluted to 0.5 OD₆₀₀ and 10 times serial dilutions of 0.5 OD₆₀₀ were dotted on SC-URA+Gal plates containing different concentration of FeSO₄, ZnCl₂, CoCl₂, CdCl₂ and MnCl₂. The plates were incubated at 30 °C for 3 days. OsCCX2 was able to suppress the Fe²⁺, Zn²⁺ and Co²⁺ sensitivity of K667 yeast mutant and hence provided tolerance towards these metals ions. OsCCX2 does not provide tolerance against excess CdCl₂ and MnCl₂.