Figure 6

OsCCX2 provides tolerance to mid1 mutant to excess Na⁺, Li⁺ and Fe²⁺ ions while does not complement ∆trk1∆trk2 mutant, wΔ6, under low K⁺ condition.

(A) The mid1 mutant shows sensitivity under excess (A) NaCl, (B) LiCl and (C) FeSO₄. Yeast strains transformed either with empty vector or OsCCX2 were grown in SC-Ura+Gal overnight at 30 °C and diluted to 0.5 OD₆₀₀ followed by 10 times serial dilutions of 0.5 OD₆₀₀ were dotted on SC-URA+Gal containing different concentrations of NaCl, LiCl and FeSO_4. The plates were incubated for 3 days. OsCCX2 was able to suppress the Na⁺, Li⁺ and Fe²⁺ sensitivity of mid1 mutant and hence provide tolerance toward these metal ions. (D) The high affinity K⁺ uptake in yeast W∆6 mutant is compromised. OsCCX2 was unable to complement W∆6 mutant and showed growth like vector control under low K⁺ concentrations on solid arginine-phosphate medium as described under “Material and methods” section. The plates were incubated for 5 days.