Figure 7

Total Ca²⁺, Na⁺, Fe²⁺ and Zn²⁺ accumulation in K667 yeast mutant expressing OsCCX2 under normal and excess metal concentrations.

Yeast strains transformed either with empty vector or OsCCX2 were grown without excess metal (control) or with indicated excess metal ions. The cells were harvested and samples were prepared for atomic absorption spectrophotometric analysis as described under “Material and methods” section. The total metal ion content in yeast was measured by atomic absorption spectrophotometry. Differences between mean values of treatments and controls were compared using Student’s t -tests (^*P≤0.05, ^**P≤0.01).