Figure 2

Phylogenies of the HBV strains from patient VB.7 with a typical virological breakthrough.

Patient VB.7 experienced a virological breakthrough at week 52 of the LdT treatment. The rtA181T mutant was detectable at weeks 12, 24 and 36, whereas the rtM204I mutant was detectable at week 52. The lineages carrying rtA181T and rtM204I are marked in blue and red, respectively. This coloring indicates that these two mutants were derived from independent phylogenetic origins. The pairwise difference of the subject’s viral sequences at each time point are shown on different circular rims in different colors (week 0, sky blue; week 12, light green; week 24, yellow green; week 36, orange; week 52, dark red). A significant decrease in the pairwise difference was observed at week 52 (after the virological breakthrough) compared with week 36 (before the virological breakthrough).