Table 2 The remaining percent activity of enzyme in the presence of protease inhibitor.
nsP2 pro | AGA | AGC | AGG |
|---|---|---|---|
Leupeptin | |||
 Wild Type | 91.25 ± 2.58 | 100.2 ± 10.2 | 106.2 ± 9.7 |
 Cys478Ala | 98.86 ± 8.11 | 102.5 ± 10.7 | 105.5 ± 7.0 |
 Ser482Ala | 96.85 ± 5.39 | 98.42 ± 16.22 | 99.97 ± 8.02 |
 Trp549Ala | 95.37 ± 11.19 | 93.77 ± 6.50 | 92.81 ± 8.45 |
 Trp549Phe | 92.70 ± 3.56 | 91.84 ± 8.04 | 90.53 ± 3.80 |
E-64 | |||
 Wild Type | 93.09 ± 2.66 | 104.1 ± 5.6 | 108.0 ± 6.0 |
 Cys478Ala | 98.73 ± 8.81 | 104.7 ± 8.3 | 107.4 ± 1.4 |
 Ser482Ala | 109.9 ± 13.9 | 95.98 ± 5.48 | 105.2 ± 8.4 |
 Trp549Ala | 102.0 ± 8.10 | 98.57 ± 7.27 | 95.43 ± 8.44 |
 Trp549Phe | 105.1 ± 5.20 | 96.35 ± 4.93 | 107.1 ± 6.15 |
Chymostatin | |||
 Wild Type | 84.03 ± 7.77 | 87.43 ± 0.09 | 90.42 ± 8.16 |
 Cys478Ala | 98.57 ± 9.98 | 67.11 ± 26.39 | 63.78 ± 30.29 |
 Ser482Ala | 123.2 ± 7.1 | 60.90 ± 17.90 | 51.57 ± 11.61 |
 Trp549Ala | 87.88 ± 2.77 | 92.60 ± 4.00 | 105 ± 13.85 |
 Trp549Phe | 81.29 ± 3.97 | 83.72 ± 2.54 | 80.79 ± 14.51 |
PMSF | |||
 Wild Type | 107.7 ± 4.3 | 93.07 ± 4.26 | 71.30 ± 6.67 |
 Cys478Ala | 113.89 ± 63.3 | 70.82 ± 14.22 | 50.87 ± 28.14 |
 Ser482Ala | 102.8 ± 8.3 | 87.56 ± 28.71 | 88.37 ± 6.57 |
 Trp549Ala | 95.24 ± 4.27 | 100.32 ± 7.86 | 91.34 ± 8.85 |
 Trp549Phe | 100.12 ± 3.61 | 83.05 ± 7.79 | 95.13 ± 4.31 |
