Table 4 Thermal stability test at 42 °C.

From: Chikungunya nsP2 protease is not a papain-like cysteine protease and the catalytic dyad cysteine is interchangeable with a proximal serine

nsP2 pro

AGG

Wild Type

50.36 ± 1.89

Cys478Ala

53.17 ± 8.82

Ser482Ala

50.43 ± 1.71

Trp549Ala

52.86 ± 8.6

Trp549Phe

53.97 ± 4.2

  1. The percent remaining activity of nsP2 pro Wild Type and mutants after incubation at 42 °C for 10 min. The activity of each enzyme incubated at 37 °C was used as a control. Values are means ± S.D. with n ≥ 3.
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