Table 5 Substrate sequences of chikungunya virus nsP2 protease used in this study.

From: Chikungunya nsP2 protease is not a papain-like cysteine protease and the catalytic dyad cysteine is interchangeable with a proximal serine

Designated name

Cleavage site (P4–P5′)

Recognition site

AGA

nsP1/2

Nma-RAGA/GIIETK(Dnp)-OH

AGC

nsP2/3

Nma-RAGC/APSYRK(Dnp)-OH

AGG

nsP3/4

Nma-RAGG/YIFSSK(Dnp)-OH

  1. The substrate sequences used in the present study have been previously reported31 and this table is adapted from the previous report. Briefly, the three fluorescent substrates designated as AGA, AGC and AGG (as underlined) were synthesized corresponding to the scissile site sequences (shown in upper case bold text) of chikungunya virus non-structural polyprotein (nsP1/2, nsP2/3 and nsP3/4), respectively. A 2-(N-methylamino)benzoyl (Nma) fluorophore group was attached at the amino terminus and a 2,4- dinitrophenyl (Dnp) group attached to the carboxyl terminus of an additional lysine (K) residue.
Back to article page