Figure 1 | Scientific Reports

Figure 1

From: Electrophysiological and Histological Characterization of Rod-Cone Retinal Degeneration and Microglia Activation in a Mouse Model of Mucopolysaccharidosis Type IIIB

Figure 1

(a) Workflow for immunohistochemistry and cell counting. Whole retina was carefully isolated and hemisected. The temporal half was sectioned using vibrating microtome into vertical sections. The distance between each section was set at 40 μm. Dotted lines denote the schematic locations of the cuts. Sections containing the optic nerve head was processed with antibodies and florescent staining and then mounted on slides. Being zoned into five zones of equal width, the second and the fourth zones from the optic nerve were respectively designated as the “central region” and “peripheral region” and were photographed by a confocal microscope for cell counting.

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