Figure 7
Representative tile-scan micrographs of whole-mount retina processed for activated microglia (Iba-1) from (a) WT and (b) MPS IIIB mice at the 46th week.
Panels (c,d) are medium magnification views of the selected regions in (a,b), respectively. Scale bar: 500 μm. The density of activated microglial cells is higher in samples from MPS IIIB mice. High magnification views (e,f) showed that microglia displays a more ramified appearance in the WT retina and a less ramified amoeboid shape in the MPS IIIB retina. (g) Bar charts comparing the percentage retinal area occupied by Iba-1-positive cells. Data points and errors bars represent group means and standard errors, respectively. (unpaired t-test with Welch correction. Asterisk: p < 0.05). Both cell density and occupied area were found to be significantly higher in the MPS IIIB sample. (h) Micrographs of MPSIII retinal sections showing the stratification of Iba-1 positive cells, which were present in the layers of OPL and GCL-IPL. (i) Bar chart showing percentage area occupied by Iba-1-positive cells were higher specifically in the GCL-IPL layer. (unpaired t-test. Asterisk: p < 0.01).
