Figure 8
(a,b) GS-IB4 labeled retinal vasculature in whole mount WT and MPSIIIB retina at the 46th week. Scale bar: 500 μm. Insets showing a magnified view of the neo-vascular tufts. Note the presence of neo-vascular tufts throughout the vasculature in MPSIIIB retina but not in the WT retina. (c,d) GFAP labeled astrocyte (bright star-shaped cells) and end-feet of Müller cells (appeared as dim punctate staining; denote by green arrows in (c)). Morphology of astrocytes appeared similar in WT and MPSIIIB retina. There was no observable change in GFAP reactivity of Müller cells in MPSIIIB retina. (e,f) No difference was found in area occupied by GFAP positive cells. (g) Retinal sections showing GFAP reactivity was localized in the GCL layer of MPSIIIB retina
