Figure 4
Intestinal calcium waves, but not pBocs, drive the DAMP peak of backward motion.
(a) The likelihood of observing forward motion, backward motion and dwelling during DMP cycles with no detectable pBocs in egl-8(n488) and egl-8(sa47) mutants. Note that here t = 0 was defined at the trough of the aBoc. (b) Baseline propensities (empty bars, measured 20–25 sec prior to the pBoc or 20–25 sec prior to the aBoc) and peak DAMP propensities (solid bars) for backward motion of wild-type animals (data reproduced from Fig. 4 for convenience), egl-8 mutants and inx-16 mutants. Data from inx-16 mutants was separated to two groups based on whether an aBoc was detected. Error bars denote denote ± s.e.m, N(n488) = 9, N(sa47) = 4, N(ox144) = 13 and N(tm1589) = 8 animals, Double asterisks denote significant differences (p < 0.01). Single asterisks above egl-8(n488) bars denote differences from corresponding wild-type data (p < 0.05). (c) Left: the mean body length of inx-16(ox144) mutants during single- and double-trough events. Right: the fractions of detected single, double and multiple body-length troughs during the L4 stage of inx-16(ox144) mutants. (d) Same as panel (a) for cycles of inx-16(ox144) mutants where an aBoc was detected (left) or not detected (right). Inset: the post-pBoc phenotype of inx-16(ox144) mutants is denoted by the shaded area between the overlaid traces of backward motion propensities in the presence and absence of the aBoc. (e,f) Same as panels (c,d) for inx-16(tm1589) mutants. In all panels, dotted grey lines represent body-length in arbitrary units as a guide to the eye, thin lines and error bars denote mean ± s.e.m.
