Figure 1

Development of taste bud organoids from CV tissue.

(a) Isolated tongue from YC mouse (white circle area: circumvallate papilla, GFP filter, Scale bars = 1000 μm), confocal image and H&E staining of CV (white dotted line: epithelium, Scale bars = 200 μm). Nuclei stained with Hoechst 33342 (Red). (b) 3D structure of taste bud organoid grows from isolated CV (white circle area in (a) from YC mouse. 3D YFP reconstructed from 1, 3, 5 or 7 day cultures. The white dotted line shows morphology of isolated CV epithelium. Scale bars = 100 μm (c) After 10 days in culture, taste bud organoids were broken up to single cells by trypsin/EDTA and re-embedded in Matrigel. Images were taken 0, 3, 5 or 7 days after passage. (d) Stem/progenitor cell or taste bud lineage marker mRNA was determined 1, 3, 5, 7, or 12 days after passage. W: water.